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Inverted Widefield Microscope with LED illumination Zeiss Axio Observer inverted microscope with a fully motorised stage controlled by Zen acquisition software. This system is also equipped with a large range of filter sets, LED light sources from UV to far-red, and a highly sensitive Hamamatsu Flash 4 camera for fast, low-light imaging

Advantages of this microscope

• Excellent for object tracking
• Easy to use
• Slide, dish and multiwell formats
• Sensitive, fast and long-term live experiments with little photodamage
• Multi-point / multiwell 4D imaging

Location

Sir Alexander Fleming building, SAF 409

Quickstart guide

Quickstart guide - Widefield 1 Zeiss (PDF)

WF1 Live Imaging Guide (PDF)‌

Widefield Tiling Method (wf1) (PDF)

Sample Carrier Setup (PDF)‌

Multi-position acquisition (PDF)

Stitching in ZEN Blue (PDF)

Hardware configuration

Please see the 

Flash Camera Data (PDF)

Frame Size Calculator (XLS)

Filter and LED setup WF1 (PDF)

Zeiss Objective List (PDF)

Offline software

• FIJI