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Citation

BibTex format

@article{Khan:2026:10.1107/S1600577526001402,
author = {Khan, S and Albers, J and Vorobyev, A and Zhang, Y and Reichmann, J and Svetlove, A and De, Marco F and Denisova, K and Yang, Y and Seichepine, F and Douglas, JO and Duke, E and Cloetens, P and Pacureanu, A and Schaefer, AT and Bosch, C},
doi = {10.1107/S1600577526001402},
journal = {J Synchrotron Radiat},
title = {Critical point drying of brain tissue for X-ray phase-contrast imaging.},
url = {http://dx.doi.org/10.1107/S1600577526001402},
year = {2026}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - X-ray phase-contrast tomography can efficiently image brain tissue at subcellular resolution. However, current sample preparation methods are not optimized to exploit the full potential of X-ray contrast mechanisms. Here we propose to replace interstitial material by air to enhance X-ray phase contrast of the ultrastructural features. Critical point drying (CPD) of heavy-metal-stained mouse brain tissue produced samples with preserved ultrastructure, a nanofoam-like material that remains compatible with follow-up conventional resin embedding. Using two synchrotron-based setups, namely, a high-throughput microtomography beamline and a nanoscale holographic tomography beamline, we found that CPD samples consistently showed 2-4× stronger phase-shift signal than samples embedded in resin. CPD offers a versatile route for preparing tissue for subcellular and ultrastructural-resolution X-ray imaging. It retains structural detail while improving signal, and is compatible with follow-up protocols involving femtosecond laser milling or electron microscopy, paving the path for biological tissue imaging beyond the mm3 scale.
AU  - Khan,S
AU  - Albers,J
AU  - Vorobyev,A
AU  - Zhang,Y
AU  - Reichmann,J
AU  - Svetlove,A
AU  - De,Marco F
AU  - Denisova,K
AU  - Yang,Y
AU  - Seichepine,F
AU  - Douglas,JO
AU  - Duke,E
AU  - Cloetens,P
AU  - Pacureanu,A
AU  - Schaefer,AT
AU  - Bosch,C
DO  - 10.1107/S1600577526001402
PY  - 2026///
TI  - Critical point drying of brain tissue for X-ray phase-contrast imaging.
T2  - J Synchrotron Radiat
UR  - http://dx.doi.org/10.1107/S1600577526001402
UR  - https://www.ncbi.nlm.nih.gov/pubmed/41871047
ER  -
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