In this section
@article{Hoddle:2026:10.1042/BCJ20260542,
author = {Hoddle, F and Schmidt, SD and Sridhar, S and Schumann, B},
doi = {10.1042/BCJ20260542},
journal = {Biochem J},
pages = {1999--2014},
title = {Design rules for bioorthogonal glycan-based tools.},
url = {http://dx.doi.org/10.1042/BCJ20260542},
volume = {483},
year = {2026}
}
TY - JOUR
AB - As one of the most abundant and diverse post-translational modifications in biology, glycosylation has a significant influence on the functions of proteins in health and disease. To untangle the complexity of glycan biosynthetic pathways, the process of studying glycosylation has been expedited by specialised, chemical labelling techniques. In the present review, we explore the development of metabolic glycan labelling from early biochemical methods to current approaches that use interdisciplinary techniques. We highlight work that has informed our understanding of glycan biosynthesis through pioneering chemical biology approaches. Moreover, we provide guidance for experimental design that can be tailored to the specific needs of those investigating glycosylation in the context of their own work. Our 'Design Rules' consolidate the findings of several groups in a simple roadmap to make metabolic glycan labelling as effective as possible.
AU - Hoddle,F
AU - Schmidt,SD
AU - Sridhar,S
AU - Schumann,B
DO - 10.1042/BCJ20260542
EP - 2014
PY - 2026///
SP - 1999
TI - Design rules for bioorthogonal glycan-based tools.
T2 - Biochem J
UR - http://dx.doi.org/10.1042/BCJ20260542
UR - https://www.ncbi.nlm.nih.gov/pubmed/42757430
VL - 483
ER -